Journal: Heliyon
Article Title: Dental stem cell sphere formation and potential for neural regeneration: A scoping review
doi: 10.1016/j.heliyon.2024.e40262
Figure Lengend Snippet: A summary of relevant in vitro & in vivo studies.
Article Snippet: 23 , Kanao, Ogura [ ] (2017) , Human/DFCSs , Low attachment , DMEM + B27, EGF, FGF Neural cell differentiation: MSC Neurogenic differentiation medium Spheres seeding: Fibronectin coating , Formation: 2 days Induction: 7 days , Test used: IF, RT-qPCR – Musashi-1, Musashi-2, MAP2, GFAP, MBP, Sox10, Nestin, β-tubulin III , NA , Spheres vs. monolayer: Nestin, Sox10, MAP2, MBP (increase) Spheres induced vs. non-induced: Musashi-1, Musashi-2, MAP2, GFAP, MBP, Sox10 (increase) Nestin, β-tubulin III (decrease) , For cell-based therapies targeting neurological illnesses, hDFCs may represent an ideal additional source of neural/glial cells..
Techniques: In Vitro, In Vivo, Marker, Expressing, Isolation, Derivative Assay, Cell Differentiation, Reverse Transcription Polymerase Chain Reaction, Concentration Assay, Immunohistochemistry, Generated, Knock-Out, Quantitative RT-PCR, Control, Membrane, Transmission Assay, Electron Microscopy, Selection, Western Blot, Activity Assay, Recombinant, Cell Culture, Microarray, Functional Assay